England have made several changes ahead of Saturday's RBS Six Nations clash against Wales at Twickenham.
There are four changes to the starting XV that defeated Italy in Rome a fortnight ago with Lancaster forced into one of the changes due to the injury to Charlie Hodgson.
Hodgson suffered a cut and sprain to his left index finger in training earlier this week and his failure to recover in time is good news for Owen Farrell, who has been named at fly-half.
Farrell's move from inside centre clears the way for Manu Tuilagi to make his England return alongside Brad Barritt.
"Charlie cut his finger in training earlier in the week and needed stitches but it swelled up and there is a sprain there," said Lancaster.
"We assessed him this morning and he cannot grip fully. Owen has trained throughout and knows our patterns and plays so we are fully confident in his ability to run the game from 10.
"We are looking forward to seeing how Brad and Manu gel together in the centre.
"It's been a good start with two wins but we've got real competition for places and Lee, Ben and Geoff deserve an opportunity to start this game."
Lancaster has handed number eight Ben Morgan, scrum-half Lee Dickson and lock Geoff Parling their first Test starts, but Toby Flood will have to make do with a place on the bench after being overlooked in favour of Farrell at fly-half.
As expected Ben Youngs and Phil Dowson have dropped to the bench while there is no place for lock Tom Palmer.
England (XV v Wales): B Foden; C Ashton, M Tuilagi, B Barritt, D Strettle; O Farrell, L Dickson; A Corbisiero, D Hartley, D Cole, M Botha, G Parling, T Croft, C Robshaw, B Morgan.
Replacements: R Webber, M Stevens, C Lawes, P Dowson, B Youngs, T Flood, M Brown.