Portugal's World Cup hopes have been dealt a massive blow with the news Nani will play no part in the competition after damaging his collarbone.
The Manchester United winger is believed to have picked up the injury in training on Friday and will be replaced in the squad by Benfica midfielder Ruben Amorim.
Nani's enforced absence will disrupt the plans of Portugal boss Carlos Queiroz, who was counting on the 23-year-old following an impressive season at Old Trafford.
A statement on the Portuguese Football Federation's official website read: "Following the injury to the left clavicle suffered by Nani, a daily assessment of the damage and the completion of medical tests, we have concluded he is unable to be competitive.
"The medical file will now be presented to the medical department of Manchester United.
"In light of the report, the Portuguese Football Federation, taking into account the FIFA regulations for the 2010 World Cup, will call-up Ruben Amorim as a replacement."